In a study published in the journal National Science Review, researchers developed an amplifiable protein identification method called „AmproCode.“
In a study published in the journal National Science Review, researchers developed an amplifiable protein identification method called „AmproCode.“
Ultrasensitive protein identification is significant but extremely challenging, because unlike the nucleic acids that could rely on the polymerase chain reaction (PCR) for amplification, proteins cannot be directly amplified.
However, the authors believe that application of a collection of residue-specific reactions that can selectively label different types of amino acids with the amplifiable DNA barcodes, in conjunction with the innovative proteome database matching method, may ultimately produce a breakthrough technique for turning proteins into amplifiable molecules. They validated their idea both computationally and experimentally in this study.
