Recent breakthroughs in single-cell RNA sequencing (scRNA), such as the recently developed « RevGel-seq » method, have revolutionized plant cell analysis. This technique, independent of special instruments, streamlines processes and resolves protoplast isolation challenges.
Recent breakthroughs in single-cell RNA sequencing (scRNA), such as the recently developed « RevGel-seq » method, have revolutionized plant cell analysis. This technique, independent of special instruments, streamlines processes and resolves protoplast isolation challenges.
Now, a multinational team of researchers review this and other recent advances in plant scRNA sequencing with the intention of providing guidance for facilitating the appropriate selection of scRNA methods for different plant samples. Their review article is published in the journal BioDesign Research.
In the world of plant biology, understanding the intricacies of individual plant cells has been a complex challenge, particularly due to the unique structure of these cells encapsulated by rigid cell walls. This challenge has, in turn, hindered the isolation of intact nuclei or protoplasts, which are essential for in-depth analysis.
Now, although traditional RNA sequencing has played an instrumental role in helping researchers understand the intricacies of plant cells, the development of single-cell RNA (scRNA) sequencing has enabled groundbreaking advancements in our understanding and analysis of plant biosystems.
In addition, other technical advancements in scRNA sequencing, such as the development of protocols for cell isolation, library preparation, and sequencing technologies have furthered researchers’ ability to overcome plant-specific challenges in analysis. Although past studies have reviewed the utility and practical applicability of scRNA sequencing, not many have attempted to look at the technological developments with regard to scRNA sequencing.
In the recent review article, researchers have comprehensively looked at the recent advances and paradigm shifts in plant systems biology. Plant systems, with their complex cellular architecture, demand innovative approaches to unravel the secrets hidden within plant cells. The review captures these recent developments in scRNA technology, and also discusses the challenges and future potential opportunities in this field.
